The ultrasonic processor | ultrasonic disperser | probe sonicator for cell crushing | cell breakage is commonly used in the laboratory to break escherichia coli, which is used to extract and express exogenous proteins and other tests
The ultrasonic processor | ultrasonic disperser | probe sonicator for cell crushing for cell crushing | cell breakage is commonly used in the laboratory to break escherichia coli, which is used to extract and express exogenous proteins and other tests. In the process of ultrasonic broken escherichia coli, the temperature of the escherichia coli bacteria liquid measured by the temperature probe is gradually increased, which may lead to the inactivation of thermally sensitive substances. How to solve this problem? Today, Let's talk about the solution.
The ultrasonic processor | ultrasonic disperser | probe sonicator for cell crushing in the treatment of e. coli in the process, as a result of ultrasonic cavitation effect will produce a large amount of heat that bacteria liquid temperature gradually raised, so we in the sample time must be dealt with in an ice bath or other cooling methods, here we give you two Suggestions, one is to do with laboratory of crushed ice ice bath, schematic diagram is as follows: ( the figure is from China Pharmaceutical University)
(the liquid in the picture is a mixture of ice water and crushed ice.)
The other method is generally suitable for the treatment of escherichia coli with 50 ml or more of ultrasound cytometry. In the picture, The ultrasonic processor | ultrasonic disperser | probe sonicator for cell crushing and the cryogenic cooling circulation device come from BuenoBiotech. You can place the bacteria solution in our special double-jacket reaction cup, and set the temperature you need on the cooling water circulation system, so that you can rest assured that you do not have to worry about the temperature rise of the bacteria solution in the experiment.
At the same time, it is very important to explore the experimental conditions in the process of treating escherichia coli. The power is adjusted according to your processing capacity. We suggest that if you can use small power to process samples, you should not use large power. The smaller the power is, the lower the thermal effect of ultrasonic wave will be.
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